In 2024, 49 startups secured funding rounds of $100 million or more, according to TechCrunch. Among them, three companies closed multiple “mega-rounds,” while seven raised rounds of $1 billion or greater.

Figure 1. 33 U.S. AI Startups Securing $100M+ Funding in 2025.

How does 2025 stack up so far? With just weeks remaining in Q3, the trend from 2024 appears to be holding strong. Several billion-dollar rounds have already closed this year, and multiple companies have secured more than one mega-round. Figure 1 shows 33 U.S. AI Startups Securing $100M+ Funding in 2025.

August

• EliseAI (healthcare & housing automation) – $250M Series E, $2.2B valuation, led by Andreessen Horowitz.
• Decart (AI research lab) – $100M, $3.1B valuation, investors include Sequoia, Benchmark, Zeev Ventures.

July

• Fal(generative media) – $125M Series C, $1.5B valuation, led by Meritech Capital.
• Ambience Healthcare – $243M Series C, AI healthcare OS, led by Oak HC/FT & Andreessen Horowitz.
• Reka AI – $110M Series B, $1B valuation, investors include Snowflake & Nvidia.
• Thinking Machines Lab – $2B seed, $12B valuation, led by Andreessen Horowitz.
• OpenEvidence – $210M Series B, $3.5B valuation, led by Kleiner Perkins & GV.
• Harmonic –$100M Series B, $875M valuation, led by Kleiner Perkins.

June

• Abridge – $300M Series E, $5.3B valuation, led by Andreessen Horowitz (second 2025 round).
• Harvey – $300M Series E, $5B valuation, co-led by Kleiner Perkins & Coatue (second 2025 round).
• Tennr – $101M Series C, $605M valuation, led by IVP.
• Glean – $150M Series F, $7.25B valuation, led by Wellington Management.
• Anysphere – $900M Series C, $10B valuation, led by Thrive Capital.

May

• Snorkel AI – $100M Series D, $1.3B valuation, led by Addition.
• LMArena – $100M seed, $600M valuation, co-led by Andreessen Horowitz & UC Investments.
• TensorWave – $100M Series A, led by Magnetar Capital & AMD Ventures.

April

• SandboxAQ –$450M Series E, $5.7B valuation, investors include Nvidia & Google.
• Runway – $308M Series D, $3B valuation, led by General Atlantic

March

• OpenAI – $40B round, $300B valuation, led by SoftBank.
• Nexthop AI – $110M Series A, led by Lightspeed Venture Partners
• Insilico Medicine – $110M Series E, $1B valuation, co-led by Value Partners & Pudong Chuangtou.
• Celestial AI –$250M Series C, $2.5B valuation, led by Fidelity.
• Lila Sciences – $200M seed, led by Flagship Pioneering.
• Reflection.Ai – $130M Series A, $580M valuation, led by Lightspeed & CRV.
• Turing – $111M Series E, $2.2B valuation, led by Khazanah Nasional.
• Shield AI – $240M Series F, $5.3B valuation, co-led by L3Harris & Hanwha Aerospace.
• Anthropic $3.5B Series E, $61.5B valuation, led by Lightspeed.

February

• Together AI – $305M Series B, $3.3B valuation, co-led by Prosperity7 & General Catalyst.
• Lambda –$480M Series D, $2.5B valuation, co-led by SGW & Andra Capital
• Abridge – $250M Series D, $2.75B valuation, co-led by IVP & Elad Gil.
• Eudia – $105M Series A, led by General Catalyst.
• EnCharge AI – $100M Series B, led by Tiger Global.
• Harvey –$300M Series D, $3B valuation, led by Sequoia.

January

• ElevenLabs –$180M Series C, $3B+ valuation, co-led by ICONIQ Growth & Andreessen Horowitz
• Hippocratic AI – $141M Series B, $1.6B+ valuation, led by Kleiner Perkins.

Source: TC

Cite this article:

Priyadharshini S (2025), 33 U.S. AI Startups Raising $100M+ in 2025, AnaTechMaz, pp. 126